Hotspot

ndhH-rps15

SSC · 129029-129142 · 114 bp

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Pi 0.0439

MarkerSeek score 64.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 5
pi: 0.0439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TCTTGTAGCCGGTACAGTCA	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.1	57.7	242 bp	1.0	78.5
ndhH-rps15_p2	TCTTGTAGCCGGTACAGTCA	TCTGGGAAAACGTCAACGAT	50.0	45.0	58.1	57.5	241 bp	1.0	76.7
ndhH-rps15_p3	TCTTGTAGCCGGTACAGTCA	ATTCTGGGAAAACGTCAACGA	50.0	42.9	58.1	58.2	243 bp	1.0	71.7
ndhH-rps15_p4	TCTTGTAGCCGGTACAGTCA	TCTGGGAAAACGTCAACGATT	50.0	42.9	58.1	58.2	241 bp	1.0	71.7
ndhH-rps15_p5	TCTTGTAGCCGGTACAGTCA	TTCTGGGAAAACGTCAACGAT	50.0	42.9	58.1	58.2	242 bp	1.0	71.7