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psaC-ndhE

SSC · 123026-123647 · 622 bp

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Pi 0.00972

MarkerSeek score 65.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 622
variable_sites: 6
pi: 0.00972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTCGAACACATTGGGTAC	TGAAGCAGCTATTGGGCTAG	50.0	50.0	57.9	57.4	773-785 bp	1.0	75.4
psaC-ndhE_p2	AGCTCGAACACATTGGGTAC	CTGAAGCAGCTATTGGGCTA	50.0	50.0	57.9	57.4	774-786 bp	1.0	75.3
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	816-828 bp	1.0	75.0
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	815-827 bp	1.0	75.0
psaC-ndhE_p5	GCTCGAACACATTGGGTACA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.2	57.4	772-784 bp	1.0	74.5