Hotspot

trnR-ACG-trnN-GUU

IRb · 113695-114241 · 547 bp

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Pi 0.011

MarkerSeek score 64.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 547
variable_sites: 6
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	688 bp	1.0	79.0
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	GTTGAGAACGGGATGAGGTC	55.0	55.0	57.8	58.0	715 bp	1.0	78.9
trnR-ACG-trnN-GUU_p3	TTTGAAGGTACTCCCGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	742 bp	1.0	78.5
trnR-ACG-trnN-GUU_p4	TTTTGAAGGTACTCCCGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	743 bp	1.0	78.5
trnR-ACG-trnN-GUU_p5	TTTGAAGGTACTCCCGGGAA	GTTGAGAACGGGATGAGGTC	50.0	55.0	58.3	58.0	769 bp	1.0	78.4