Hotspot

trnL-UAA-trnF-GAA

LSC · 50523-50914 · 392 bp

Back to Tropidia

Pi 0.0918

MarkerSeek score 86.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 36
pi: 0.0918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	482-484 bp	1.0	81.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	497-499 bp	1.0	81.3
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TTATTCACGTGCCAGGAACC	50.0	50.0	57.5	58.2	505-507 bp	1.0	80.4
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	444-446 bp	1.0	79.1
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	479-481 bp	1.0	78.5