Hotspot

atpF-atpH

LSC · 14162-14723 · 562 bp

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Pi 0.0113

MarkerSeek score 69.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 6
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAACTCGAAACTCCCAGCG	AATTTATGGGCTGGTCGTGG	50.0	50.0	57.9	58.2	656-684 bp	1.0	81.8
atpF-atpH_p2	TTAACTCGAAACTCCCAGCG	GGTCGTGGCATTAGCTCTTT	50.0	50.0	57.9	58.3	644-672 bp	1.0	81.7
atpF-atpH_p3	TTAACTCGAAACTCCCAGCG	ACAATTTATGGGCTGGTCGT	50.0	45.0	57.9	57.5	658-686 bp	1.0	79.6
atpF-atpH_p4	TTAACTCGAAACTCCCAGCG	CAATTTATGGGCTGGTCGTG	50.0	50.0	57.9	57.2	657-685 bp	1.0	76.9
atpF-atpH_p5	TAACTCGAAACTCCCAGCGA	AATTTATGGGCTGGTCGTGG	50.0	50.0	58.7	58.2	655-683 bp	1.0	76.4