Hotspot

trnS-GCU-trnG-UCC

LSC · 8789-10075 · 1287 bp

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Pi 0.0167

MarkerSeek score 58.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1287
variable_sites: 19
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	1204-1356 bp	1.0	56.9
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	1203-1355 bp	1.0	56.9
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	1205-1357 bp	1.0	54.9
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	AACGAATCACACTTTTACCACT	50.0	36.4	58.0	56.2	1204-1356 bp	1.0	54.9
trnS-GCU-trnG-UCC_p5	CGGAGAGAGAGGGATTCGAA	ACGAATCACACTTTTACCACT	55.0	38.1	58.3	55.5	1257-1409 bp	1.0	54.3