Hotspot

rps15-ycf1

SSC · 121637-122000 · 364 bp

Back to Tropaeolum

Pi 0.101

MarkerSeek score 77.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 364
variable_sites: 34
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCATTGATGGATGGTGAGG	AGGGGAGAAATGCGTCTAGA	50.0	50.0	57.9	57.8	1375-1410 bp	1.0	85.4
rps15-ycf1_p2	TGCATTGATGGATGGTGAGG	CAGTCCGTCTGGCTGTAAAA	50.0	50.0	57.9	57.8	1287-1322 bp	1.0	85.4
rps15-ycf1_p3	TGCATTGATGGATGGTGAGG	CAAAGGGGAGAAATGCGTCT	50.0	50.0	57.9	58.2	1378-1413 bp	1.0	85.3
rps15-ycf1_p4	GCATTGATGGATGGTGAGGT	AGGGGAGAAATGCGTCTAGA	50.0	50.0	57.9	57.8	1409 bp	0.5	58.2
rps15-ycf1_p5	GCATTGATGGATGGTGAGGT	CAGTCCGTCTGGCTGTAAAA	50.0	50.0	57.9	57.8	1321 bp	0.5	58.2