Hotspot

ndhF-rpl32

SSC · 110418-111383 · 966 bp

Back to Tropaeolum

Pi 0.0789

MarkerSeek score 80.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 966
variable_sites: 75
pi: 0.0789
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGCGCGGATTTAGCAATTG	TTTAACAAGTGGGCGATGGG	45.0	50.0	58.1	58.5	2009-2147 bp	1.0	81.1
ndhF-rpl32_p2	CGCGGATTTAGCAATTGGAC	TTTAACAAGTGGGCGATGGG	50.0	50.0	57.9	58.5	2006-2144 bp	1.0	81.0
ndhF-rpl32_p3	TTGATGGGCAGAACTTCTCG	TTTAACAAGTGGGCGATGGG	50.0	50.0	57.9	58.5	1490 bp	0.5	55.5
ndhF-rpl32_p4	GATGGGCAGAACTTCTCGTT	TTTAACAAGTGGGCGATGGG	50.0	50.0	57.9	58.5	1488 bp	0.5	55.5
ndhF-rpl32_p5	GGGAAATTGCGCGGATTTAG	TTTAACAAGTGGGCGATGGG	50.0	50.0	57.9	58.5	2015 bp	0.5	55.4