Hotspot

ndhC-trnV-UAC

LSC · 49245-49996 · 752 bp

Back to Tropaeolum

Pi 0.0498
MarkerSeek score 79.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
752
variable_sites
36
pi
0.0498
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGT GGTTCGAATCCGTATAGCCC 34.8 55.0 56.1 57.9 810-814 bp 1.0 46.7
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGT CGGTTCGAATCCGTATAGCC 34.8 55.0 56.1 58.3 811-815 bp 1.0 46.4
ndhC-trnV-UAC_p3 ATGCCCAGAAAATATCATATTCGT GGTTCGAATCCGTATAGCCC 33.3 55.0 56.6 57.9 811-815 bp 1.0 46.4
ndhC-trnV-UAC_p4 TGCCCAGAAAATATCATATTCG GGTTCGAATCCGTATAGCCC 36.4 55.0 54.4 57.9 810-814 bp 1.0 46.4
ndhC-trnV-UAC_p5 GCCCAGAAAATATCATATTCGT GGTTCGAATCCGTATAGCCC 36.4 55.0 54.4 57.9 809-813 bp 1.0 46.4