Hotspot

trnS-GGA-rps4

LSC · 44181-44476 · 296 bp

Back to Tropaeolum

Pi 0.0616

MarkerSeek score 78.70

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 17
pi: 0.0616
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGGAAATGGGTCGGTTTGAA	50.0	45.0	58.1	57.3	391-402 bp	1.0	81.3
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	AGGAAATGGGTCGGTTTGAA	50.0	45.0	58.4	57.3	390-401 bp	1.0	79.0
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	AGGAAATGGGTCGGTTTGAAA	50.0	42.9	58.1	58.0	391-402 bp	1.0	78.3
trnS-GGA-rps4_p4	GGCTTTTGTTTACCGAGGGT	AGGAAATGGGTCGGTTTGAAA	50.0	42.9	58.4	58.0	390-401 bp	1.0	76.0
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	GGAAATGGGTCGGTTTGAAA	50.0	45.0	58.1	56.5	390-401 bp	1.0	74.9