Hotspot

psaA-pafI

LSC · 41199-41903 · 705 bp

Back to Tropaeolum

Pi 0.0449

MarkerSeek score 79.30

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 31
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TTACGGGATCCCTATCGACC	GGAAACAGGCTATAGCGCTT	55.0	50.0	58.1	58.0	829-843 bp	1.0	83.4
psaA-pafI_p2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	870-884 bp	1.0	83.1
psaA-pafI_p3	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	851-865 bp	1.0	83.0
psaA-pafI_p4	TTACGGGATCCCTATCGACC	AATTGCGGAGGCTTGGTTTA	55.0	45.0	58.1	58.1	867-881 bp	1.0	82.9
psaA-pafI_p5	TGAGAAATGACCCGGTTTGG	AATTGCGGAGGCTTGGTTTA	50.0	45.0	58.1	58.1	908-922 bp	1.0	82.6