Hotspot

psbK-psbI

LSC · 7051-7478 · 428 bp

Back to Tropaeolum

Pi 0.0742

MarkerSeek score 78.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 31
pi: 0.0742
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GGAATCCGAAGATGAAGAGGG	50.0	52.4	57.9	57.9	526-528 bp	1.0	76.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	CCGAAGATGAAGAGGGAAACA	50.0	47.6	57.9	57.7	521-523 bp	1.0	75.2
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GGAATCCGAAGATGAAGAGGG	45.0	52.4	58.3	57.9	523-525 bp	1.0	74.8
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GGAATCCGAAGATGAAGAGGG	45.0	52.4	58.3	57.9	524-526 bp	1.0	74.8
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GGAATCCGAAGATGAAGAGGG	45.0	52.4	58.3	57.9	525-527 bp	1.0	74.8