primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	AAAAAGGGGTTTGTTGCTGC	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.052693	4219	4933	1553-1639	1.000000	84.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AAAGGGGTTTGTTGCTGCTA	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.096390	4219	4933	1551-1637	1.000000	84.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGGATCAGTCGTGGTCTT	AAAAAGGGGTTTGTTGCTGC	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.112226	4219	4933	1549-1635	1.000000	84.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TTTGTGCCGATTCGAAAACG	AAAAAGGGGTTTGTTGCTGC	20	20	45.0	45.0	58.0	58.0	17.1	0.0	0.062502	4219	4933	1640-1640	0.500000	59.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TTTGTGCCGATTCGAAAACG	AAAGGGGTTTGTTGCTGCTA	20	20	45.0	45.0	58.0	57.9	17.1	0.0	0.106198	4219	4933	1638-1638	0.500000	59.2
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	GGAATCCGAAGATGAAGAGGG	20	21	50.0	52.4	57.9	57.9	1.4	0.0	1.174999	7051	7478	526-528	1.000000	76.8
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	CCGAAGATGAAGAGGGAAACA	20	21	50.0	47.6	57.9	57.7	1.4	0.0	1.390677	7051	7478	521-523	1.000000	75.2
psbK-psbI_p3	psbK-psbI	3	TGGCAAGCTGCTGTAAGTTT	GGAATCCGAAGATGAAGAGGG	20	21	45.0	52.4	58.3	57.9	1.4	0.0	1.427709	7051	7478	523-525	1.000000	74.8
psbK-psbI_p4	psbK-psbI	4	TTGGCAAGCTGCTGTAAGTT	GGAATCCGAAGATGAAGAGGG	20	21	45.0	52.4	58.3	57.9	1.4	0.0	1.427709	7051	7478	524-526	1.000000	74.8
psbK-psbI_p5	psbK-psbI	5	TTTGGCAAGCTGCTGTAAGT	GGAATCCGAAGATGAAGAGGG	20	21	45.0	52.4	58.3	57.9	1.4	0.0	1.427709	7051	7478	525-527	1.000000	74.8
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	GGGCGAATCACACTTTTACC	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.918360	7769	8299	602-642	1.000000	79.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	GGGCGAATCACACTTTTACC	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.919748	7769	8299	601-641	1.000000	79.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGGAAACGGAAAGAGAGGGA	GGGCGAATCACACTTTTACC	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.939413	7769	8299	661-701	1.000000	78.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	GGGCGAATCACACTTTTACC	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.065125	7769	8299	656-696	1.000000	78.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	GGCGAATCACACTTTTACCAC	20	21	50.0	47.6	58.0	57.8	0.0	0.0	1.202016	7769	8299	601-641	1.000000	77.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	27163	27825	719-741	1.000000	83.4
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	27163	27825	737-759	1.000000	81.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	27163	27825	718-740	1.000000	77.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	CCAGTTCAAATCCGGGTGTC	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.8	58.2	0.0	0.0	0.986079	27163	27825	714-736	1.000000	77.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	27163	27825	727-749	1.000000	77.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	30272	30880	713-726	1.000000	85.0
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	30272	30880	692-705	1.000000	84.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	30272	30880	689-702	1.000000	84.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30272	30880	691-704	1.000000	84.4
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	30272	30880	710-723	1.000000	83.9
psaA-pafI_p1	psaA-pafI	1	TTACGGGATCCCTATCGACC	GGAAACAGGCTATAGCGCTT	20	20	55.0	50.0	58.1	58.0	28.4	18.7	0.129932	41199	41903	829-843	1.000000	83.4
psaA-pafI_p2	psaA-pafI	2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.140786	41199	41903	870-884	1.000000	83.1
psaA-pafI_p3	psaA-pafI	3	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	41199	41903	851-865	1.000000	83.0
psaA-pafI_p4	psaA-pafI	4	TTACGGGATCCCTATCGACC	AATTGCGGAGGCTTGGTTTA	20	20	55.0	45.0	58.1	58.1	28.4	0.0	0.166987	41199	41903	867-881	1.000000	82.9
psaA-pafI_p5	psaA-pafI	5	TGAGAAATGACCCGGTTTGG	AATTGCGGAGGCTTGGTTTA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.177841	41199	41903	908-922	1.000000	82.6
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	AGGAAATGGGTCGGTTTGAA	20	20	50.0	45.0	58.1	57.3	0.0	0.0	0.762506	44181	44476	391-402	1.000000	81.3
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	GGCTTTTGTTTACCGAGGGT	AGGAAATGGGTCGGTTTGAA	20	20	50.0	45.0	58.4	57.3	0.0	0.0	1.055467	44181	44476	390-401	1.000000	79.0
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	AGGCTTTTGTTTACCGAGGG	AGGAAATGGGTCGGTTTGAAA	20	21	50.0	42.9	58.1	58.0	0.0	0.0	1.142246	44181	44476	391-402	1.000000	78.3
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	GGCTTTTGTTTACCGAGGGT	AGGAAATGGGTCGGTTTGAAA	20	21	50.0	42.9	58.4	58.0	0.0	0.0	1.435207	44181	44476	390-401	1.000000	76.0
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	AGGCTTTTGTTTACCGAGGG	GGAAATGGGTCGGTTTGAAA	20	20	50.0	45.0	58.1	56.5	0.0	0.0	1.573563	44181	44476	390-401	1.000000	74.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47040	47601	579-674	1.000000	87.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	47040	47601	578-673	1.000000	87.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	TGATGCTTCGGTAATGGTCG	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.103798	47040	47601	634-729	1.000000	86.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	TGATGCTTCGGTAATGGTCG	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.103798	47040	47601	633-728	1.000000	86.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.243673	47040	47601	620-715	1.000000	85.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCCAGAAAATATCATATTCGT	GGTTCGAATCCGTATAGCCC	23	20	34.8	55.0	56.1	57.9	0.0	0.0	4.957231	49245	49996	810-814	1.000000	46.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCCAGAAAATATCATATTCGT	CGGTTCGAATCCGTATAGCC	23	20	34.8	55.0	56.1	58.3	0.0	22.8	5.186613	49245	49996	811-815	1.000000	46.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATGCCCAGAAAATATCATATTCGT	GGTTCGAATCCGTATAGCCC	24	20	33.3	55.0	56.6	57.9	0.0	0.0	5.474345	49245	49996	811-815	1.000000	46.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCCAGAAAATATCATATTCG	GGTTCGAATCCGTATAGCCC	22	20	36.4	55.0	54.4	57.9	0.0	0.0	5.680733	49245	49996	810-814	1.000000	46.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GCCCAGAAAATATCATATTCGT	GGTTCGAATCCGTATAGCCC	22	20	36.4	55.0	54.4	57.9	0.0	0.0	5.684147	49245	49996	809-813	1.000000	46.4
psbE-petL_p1	psbE-petL	1	TCAGCCATTTATCCCGCTTC	TGACCTCCCGCGAATTAAAG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.051223	62882	64119	2335-2343	1.000000	84.3
psbE-petL_p2	psbE-petL	2	TCAGCCATTTATCCCGCTTC	GCGGTAAGGAACTGTCGAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.038652	62882	64119	2070-2070	0.500000	59.7
psbE-petL_p3	psbE-petL	3	TCGTTCGGGTTTGATTGTGT	GCGGTAAGGAACTGTCGAAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.049674	62882	64119	1827-1827	0.500000	59.6
psbE-petL_p4	psbE-petL	4	ATGGAATTCCTTGTCGGCTC	GCGGTAAGGAACTGTCGAAT	20	20	50.0	50.0	58.0	58.0	24.7	0.0	0.050093	62882	64119	1596-1596	0.500000	59.6
psbE-petL_p5	psbE-petL	5	CGGTAGGTACAGCTAGTCCA	GCGGTAAGGAACTGTCGAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.054415	62882	64119	1737-1737	0.500000	59.6
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	CCTGCTAAAGAACCGAACCA	20	20	45.0	50.0	57.9	57.8	5.7	0.0	0.292822	64946	65289	450-456	1.000000	82.6
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	AAAACAAACGCGCTACCAAG	ACCTGCTAAAGAACCGAACC	20	20	45.0	50.0	57.9	57.8	5.7	0.0	0.294342	64946	65289	451-457	1.000000	82.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	AAGGATCCCTCGTTACTGCT	CCTGCTAAAGAACCGAACCA	20	20	50.0	50.0	58.1	57.8	8.6	0.0	0.311549	64946	65289	527-533	1.000000	81.7
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AAGGATCCCTCGTTACTGCT	ACCTGCTAAAGAACCGAACC	20	20	50.0	50.0	58.1	57.8	8.6	0.0	0.313069	64946	65289	528-534	1.000000	81.7
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ATCCCTCGTTACTGCTCAGA	CCTGCTAAAGAACCGAACCA	20	20	50.0	50.0	57.9	57.8	3.6	0.0	0.324309	64946	65289	523-529	1.000000	81.7
ndhF-rpl32_p1	ndhF-rpl32	1	TTGCGCGGATTTAGCAATTG	TTTAACAAGTGGGCGATGGG	20	20	45.0	50.0	58.1	58.5	8.8	0.0	0.552976	110418	111383	2009-2147	1.000000	81.1
ndhF-rpl32_p2	ndhF-rpl32	2	CGCGGATTTAGCAATTGGAC	TTTAACAAGTGGGCGATGGG	20	20	50.0	50.0	57.9	58.5	7.7	0.0	0.565056	110418	111383	2006-2144	1.000000	81.0
ndhF-rpl32_p3	ndhF-rpl32	3	TTGATGGGCAGAACTTCTCG	TTTAACAAGTGGGCGATGGG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.558874	110418	111383	1490-1490	0.500000	55.5
ndhF-rpl32_p4	ndhF-rpl32	4	GATGGGCAGAACTTCTCGTT	TTTAACAAGTGGGCGATGGG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.560338	110418	111383	1488-1488	0.500000	55.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGGAAATTGCGCGGATTTAG	TTTAACAAGTGGGCGATGGG	20	20	50.0	50.0	57.9	58.5	18.0	0.0	0.579404	110418	111383	2015-2015	0.500000	55.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ATGTGAATGGGGATGGGGAT	TTTAGTGGGGTCCTCCGATT	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.489428	111543	112470	1565-1565	0.500000	56.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ATGTGAATGGGGATGGGGAT	TTTTAGTGGGGTCCTCCGAT	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.489428	111543	112470	1566-1566	0.500000	56.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ATGTGAATGGGGATGGGGAT	ACTGAGATTGGAGAGCAGGT	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.500762	111543	112470	1982-1982	0.500000	56.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ATGTGAATGGGGATGGGGAT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.574838	111543	112470	1438-1438	0.500000	55.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ATGTGAATGGGGATGGGGAT	GACCCGAAGTAGCAAAACCT	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.630993	111543	112470	1928-1928	0.500000	55.0
rps15-ycf1_p1	rps15-ycf1	1	TGCATTGATGGATGGTGAGG	AGGGGAGAAATGCGTCTAGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.222230	121637	122000	1375-1410	1.000000	85.4
rps15-ycf1_p2	rps15-ycf1	2	TGCATTGATGGATGGTGAGG	CAGTCCGTCTGGCTGTAAAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.225327	121637	122000	1287-1322	1.000000	85.4
rps15-ycf1_p3	rps15-ycf1	3	TGCATTGATGGATGGTGAGG	CAAAGGGGAGAAATGCGTCT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.235124	121637	122000	1378-1413	1.000000	85.3
rps15-ycf1_p4	rps15-ycf1	4	GCATTGATGGATGGTGAGGT	AGGGGAGAAATGCGTCTAGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.223691	121637	122000	1409-1409	0.500000	58.2
rps15-ycf1_p5	rps15-ycf1	5	GCATTGATGGATGGTGAGGT	CAGTCCGTCTGGCTGTAAAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.226789	121637	122000	1321-1321	0.500000	58.2
ycf1_p1	ycf1	1	ATGGGGATATCCACCAGCAT	ATAGAGCCACCCAACACTGT	20	20	50.0	50.0	58.2	58.6	26.4	0.0	0.873448	123502	123621	268-268	1.000000	80.8
ycf1_p2	ycf1	2	ATGGGGATATCCACCAGCAT	AATAGAGCCACCCAACACTG	20	20	50.0	50.0	58.2	57.5	26.4	0.0	0.732571	123502	123621	269-269	0.500000	54.1
ycf1_p3	ycf1	3	ATGGGGATATCCACCAGCAT	CTGCCCTTCAATATCACGGA	20	20	50.0	50.0	58.2	57.4	26.4	0.0	0.855924	123502	123621	475-475	0.500000	53.2
ycf1_p4	ycf1	4	ATGGGGATATCCACCAGCAT	TCTGCCCTTCAATATCACGG	20	20	50.0	50.0	58.2	57.4	26.4	0.0	0.855924	123502	123621	476-476	0.500000	53.2
ycf1_p5	ycf1	5	ATGGGGATATCCACCAGCAT	GCAAAATAGAGCCACCCAAC	20	20	50.0	50.0	58.2	57.3	26.4	0.0	0.890422	123502	123621	273-273	0.500000	52.9