Hotspot

ndhF

SSC · 115108-117336 · 2229 bp

Back to Trollius

Pi 0.00583

MarkerSeek score 39.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 47
pi: 0.00583
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2579-2645 bp	1.0	86.0
ndhF_p2	AAGAGGGATCCACCGAAGAA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2571-2637 bp	1.0	86.0
ndhF_p3	CCTTCCCCTTCCCTTTGTTC	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2548-2614 bp	1.0	85.8
ndhF_p4	TTTAGGCAGAATACCGTCGC	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.1	57.9	2681-2747 bp	1.0	85.5
ndhF_p5	GGGGACTGAACAGGAACAAG	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2588-2654 bp	1.0	85.5