Hotspot

ndhH-rps15

SSC · 128323-128463 · 141 bp

Back to Trollius

Pi 0.0241

MarkerSeek score 65.00

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 11
pi: 0.0241
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GAGTCGAAGAACACCATGCA	CGGGAGCCAAAAACTCGTTA	50.0	50.0	58.2	58.5	232-256 bp	1.0	82.1
ndhH-rps15_p2	TGAGTCGAAGAACACCATGC	CGGGAGCCAAAAACTCGTTA	50.0	50.0	58.2	58.5	233-257 bp	1.0	82.1
ndhH-rps15_p3	AGTCGAAGAACACCATGCAT	CGGGAGCCAAAAACTCGTTA	45.0	50.0	57.5	58.5	231-255 bp	1.0	79.9
ndhH-rps15_p4	GAGTCGAAGAACACCATGCA	TTCTGGGAAAACGTCAACGG	50.0	50.0	58.2	58.7	318-342 bp	1.0	79.3
ndhH-rps15_p5	TGAGTCGAAGAACACCATGC	TTCTGGGAAAACGTCAACGG	50.0	50.0	58.2	58.7	319-343 bp	1.0	79.3