Hotspot

rpl32-trnL-UAG

SSC · 118474-119500 · 1027 bp

Back to Trollius

Pi 0.0165

MarkerSeek score 72.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1027
variable_sites: 52
pi: 0.0165
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAAATCACTTTCCACCGGGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	822-1154 bp	1.0	87.0
rpl32-trnL-UAG_p2	CAAATCACTTTCCACCGGGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	823-1155 bp	1.0	87.0
rpl32-trnL-UAG_p3	CAAATCACTTTCCACCGGGA	TTGATAAGATGCCGCCACTC	50.0	50.0	58.1	58.0	882-1214 bp	1.0	86.5
rpl32-trnL-UAG_p4	CAAATCACTTTCCACCGGGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	832-1164 bp	1.0	86.0
rpl32-trnL-UAG_p5	CAAATCACTTTCCACCGGGA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.1	57.8	830-1162 bp	1.0	85.5