Hotspot

ndhF-rpl32

SSC · 117337-118299 · 963 bp

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Pi 0.0134

MarkerSeek score 71.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 963
variable_sites: 43
pi: 0.0134
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAACTGGAAGGGGAATGA	ACGAATACGCTTTTTGGACA	50.0	40.0	57.9	56.0	1043-1098 bp	1.0	70.7
ndhF-rpl32_p2	GAGAAGGCCCAACCCTATTG	ACGAATACGCTTTTTGGACA	55.0	40.0	57.9	56.0	1067-1122 bp	1.0	70.6
ndhF-rpl32_p3	AATACTTTTTGTTGCCGCCG	ACGAATACGCTTTTTGGACA	45.0	40.0	57.9	56.0	1094-1149 bp	1.0	70.4
ndhF-rpl32_p4	GAGAAGGCCCAACCCTATTG	GAAAGCCTTTATTGATGCCCA	55.0	42.9	57.9	56.8	1115-1170 bp	1.0	69.0
ndhF-rpl32_p5	AATACTTTTTGTTGCCGCCG	GAAAGCCTTTATTGATGCCCA	45.0	42.9	57.9	56.8	1142-1197 bp	1.0	68.8