Hotspot

ycf1-ndhF

SSC · 115059-115107 · 49 bp

Back to Trollius

Pi 0.261

MarkerSeek score 68.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 49
variable_sites: 31
pi: 0.261
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.0	58.1	392-448 bp	1.0	91.2
ycf1-ndhF_p2	AAGAGGGATCCACCGAAGAA	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.0	58.1	384-440 bp	1.0	91.1
ycf1-ndhF_p3	TTTAGGCAGAATACCGTCGC	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.1	58.1	494-550 bp	1.0	91.0
ycf1-ndhF_p4	CCTTCCCCTTCCCTTTGTTC	GGGGGCGTATCTCCTCTTAT	55.0	55.0	58.1	58.1	361-417 bp	1.0	90.9
ycf1-ndhF_p5	GGGGACTGAACAGGAACAAG	GGGGGCGTATCTCCTCTTAT	55.0	55.0	58.1	58.1	401-457 bp	1.0	90.7