Hotspot

rpl14-rpl16

LSC · 85355-85497 · 143 bp

Back to Trollius

Pi 0.0193

MarkerSeek score 63.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 143
variable_sites: 9
pi: 0.0193
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ATCGGCGATTACTAGCTCCT	GCGTCCAAAATGCCTATACG	50.0	50.0	58.1	57.3	266-285 bp	1.0	81.2
rpl14-rpl16_p2	ATCGGCGATTACTAGCTCCT	TGAAATGGGCGGAGTATCAG	50.0	50.0	58.1	57.4	318-337 bp	1.0	81.0
rpl14-rpl16_p3	ATTCTCTAGCCCCGCTGTTA	TGAAATGGGCGGAGTATCAG	50.0	50.0	58.2	57.4	279-298 bp	1.0	80.4
rpl14-rpl16_p4	TAATTCTCTAGCCCCGCTGT	TGAAATGGGCGGAGTATCAG	50.0	50.0	58.2	57.4	281-300 bp	1.0	80.4
rpl14-rpl16_p5	TTCTCTAGCCCCGCTGTTAT	TGAAATGGGCGGAGTATCAG	50.0	50.0	58.2	57.4	278-297 bp	1.0	80.4