Hotspot

ndhC-trnV-UAC

LSC · 54231-55751 · 1521 bp

Back to Trollius

Pi 0.0248

MarkerSeek score 71.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1521
variable_sites: 111
pi: 0.0248
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	1364-1593 bp	1.0	57.2
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.1	57.5	1363-1592 bp	1.0	54.4
ndhC-trnV-UAC_p3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1352-1581 bp	1.0	53.3
ndhC-trnV-UAC_p4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.6	57.8	1363-1592 bp	1.0	53.2
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.6	57.8	1364-1593 bp	1.0	53.2