Hotspot

ycf3-trnS-GGA

LSC · 46962-47857 · 896 bp

Back to Trollius

Pi 0.00938

MarkerSeek score 63.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 28
pi: 0.00938
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	TGTTCGTGAATTAGGCGAGG	47.6	50.0	58.0	58.0	1004-1037 bp	1.0	76.5
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	961-994 bp	1.0	76.0
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	982-1015 bp	1.0	75.4
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	CGTGAATTAGGCGAGGGTAC	47.6	55.0	58.0	58.2	1000-1033 bp	1.0	75.3
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GTGAATTAGGCGAGGGTACG	47.6	55.0	58.0	58.2	999-1032 bp	1.0	75.3