Hotspot

trnT-GGU-psbD

LSC · 33700-35283 · 1584 bp

Back to Trollius

Pi 0.00622

MarkerSeek score 52.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1584
variable_sites: 42
pi: 0.00622
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1727-1772 bp	1.0	81.0
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1726-1771 bp	1.0	79.8
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1690-1735 bp	1.0	79.7
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	TCCCTCCGTAACCAGTCATC	50.0	55.0	57.9	58.5	1712-1757 bp	1.0	78.9
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.3	57.6	1689-1734 bp	1.0	78.5