Hotspot

rps16-trnQ-UUG

LSC · 6178-7419 · 1242 bp

Back to Trollius

Pi 0.0104
MarkerSeek score 60.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1242
variable_sites
46
pi
0.0104
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1308-1361 bp 1.0 84.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1287-1340 bp 1.0 83.9
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1298-1351 bp 1.0 83.3
rps16-trnQ-UUG_p4 CCACAACCGATCATGTCCTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1312-1365 bp 1.0 82.9
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1291-1344 bp 1.0 82.8