Hotspot

trnK-UUU-rps16

LSC · 4325-5023 · 699 bp

Back to Trollius

Pi 0.016

MarkerSeek score 55.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 31
pi: 0.016
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGGATCAGTCGTGGTCTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.1	58.2	905-1000 bp	1.0	84.2
trnK-UUU-rps16_p2	TCAGGATCAGTCGTGGTCTT	ATTCTTTCTCGAGCCGTACG	50.0	50.0	58.1	57.8	1112-1207 bp	1.0	83.5
trnK-UUU-rps16_p3	AAAGCAGGGGATTTCTTGGG	CCTTGAAAAAGGCGCTCAAC	50.0	50.0	58.1	58.2	1515-1610 bp	1.0	82.6
trnK-UUU-rps16_p4	AAAGCAGGGGATTTCTTGGG	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.1	58.2	1514-1609 bp	1.0	82.6
trnK-UUU-rps16_p5	AAAGCAGGGGATTTCTTGGG	ATTCTTTCTCGAGCCGTACG	50.0	50.0	58.1	57.8	1721-1816 bp	1.0	82.4