Hotspot

trnH-GUG-psbA

LSC · 106-412 · 307 bp

Back to Trollius

Pi 0.0179

MarkerSeek score 56.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 307
variable_sites: 25
pi: 0.0179
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	382-425 bp	1.0	87.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	396-439 bp	1.0	87.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	395-438 bp	1.0	87.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	397-440 bp	1.0	87.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TATCATCAACCGCGCTAACC	55.0	50.0	58.1	58.1	393-436 bp	1.0	86.7