Hotspot

rps8

LSC · 84786-85190 · 405 bp

Back to Triumfetta

Pi 0.00494

MarkerSeek score 40.20

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 5
pi: 0.00494
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AGTTTCGGCTACAATTCGGA	TCGGAAGCAACAATAATTTTAA	45.0	27.3	57.2	52.7	464 bp	1.0	40.9
rps8_p2	AAGTTTCGGCTACAATTCGGA	TCGGAAGCAACAATAATTTTAA	42.9	27.3	58.0	52.7	465 bp	1.0	40.9
rps8_p3	AGTTTCGGCTACAATTCGGA	TCGGAAGCAACAATAATTTTAAT	45.0	26.1	57.2	53.3	464 bp	1.0	40.9
rps8_p4	AGTTTCGGCTACAATTCGGAT	TCGGAAGCAACAATAATTTTAA	42.9	27.3	57.7	52.7	464 bp	1.0	40.9
rps8_p5	AGTTTCGGCTACAATTCGGA	TCGGAAGCAACAATAATTTTAATT	45.0	25.0	57.2	54.1	464 bp	1.0	40.9