Hotspot

psbD

LSC · 34677-35738 · 1062 bp

Back to Triumfetta

Pi 0.00484

MarkerSeek score 44.50

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 18
pi: 0.00484
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	ATTATAAGGGGCAGTGCACG	ACGACCGGCTAAAGCTAAAG	50.0	50.0	58.0	58.0	1201 bp	1.0	80.9
psbD_p2	ATTATAAGGGGCAGTGCACG	CGACCGGCTAAAGCTAAAGT	50.0	50.0	58.0	58.0	1200 bp	1.0	80.9
psbD_p3	CCAAACGCCCCCATAACATA	ACGACCGGCTAAAGCTAAAG	50.0	50.0	57.9	58.0	1150 bp	1.0	80.7
psbD_p4	CCAAACGCCCCCATAACATA	CGACCGGCTAAAGCTAAAGT	50.0	50.0	57.9	58.0	1149 bp	1.0	80.7
psbD_p5	TGCTCGGAAATGGTTGAAGT	CGACCGGCTAAAGCTAAAGT	45.0	50.0	57.7	58.0	1126 bp	1.0	79.1