Hotspot

psaC-ndhD

SSC · 126850-126995 · 146 bp

Back to Triumfetta

Pi 0.0398

MarkerSeek score 57.30

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 146
variable_sites: 13
pi: 0.0398
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	GAATCCGCTTGTCCAACAGA	ACCTGCAAATACTGGTAAAACT	50.0	36.4	58.2	55.9	284-285 bp	1.0	49.3
psaC-ndhD_p2	TCCGCTTGTCCAACAGATTT	ACCTGCAAATACTGGTAAAACT	45.0	36.4	57.7	55.9	281-282 bp	1.0	48.7
psaC-ndhD_p3	GAATCCGCTTGTCCAACAGA	GAACCTGCAAATACTGGTAAAACT	50.0	37.5	58.2	57.7	286-287 bp	1.0	47.5
psaC-ndhD_p4	GAATCCGCTTGTCCAACAGA	AACCTGCAAATACTGGTAAAACT	50.0	34.8	58.2	56.6	285-286 bp	1.0	47.0
psaC-ndhD_p5	TCCGCTTGTCCAACAGATTT	GAACCTGCAAATACTGGTAAAACT	45.0	37.5	57.7	57.7	283-284 bp	1.0	46.9