Hotspot

rpl33-rps18

LSC · 71710-72328 · 619 bp

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Pi 0.0131

MarkerSeek score 55.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 16
pi: 0.0131
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACATACAATTCACGGGGAGA	GCAAACGCCTACGAAAAGAC	45.0	50.0	56.2	58.1	663-713 bp	1.0	71.3
rpl33-rps18_p2	ACATACAATTCACGGGGAGA	CTACGAAAAGACCGCTTGGA	45.0	50.0	56.2	57.9	655-705 bp	1.0	71.2
rpl33-rps18_p3	ACATACAATTCACGGGGAGA	ACGAAAAGACCGCTTGGATT	45.0	45.0	56.2	58.1	653-703 bp	1.0	70.9
rpl33-rps18_p4	ACATACAATTCACGGGGAGA	TCAATTCGATCCCCCGATTG	45.0	50.0	56.2	57.7	691-741 bp	1.0	69.7
rpl33-rps18_p5	AACATACAATTCACGGGGAGA	GCAAACGCCTACGAAAAGAC	42.9	50.0	57.0	58.1	664-714 bp	1.0	69.5