Hotspot

psbE-petL

LSC · 68805-69658 · 854 bp

Back to Triumfetta

Pi 0.00591

MarkerSeek score 53.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 15
pi: 0.00591
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	57.8	57.6	939-940 bp	1.0	52.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GCTGAGGTTATAGTTAAAGCTGC	55.0	43.5	58.2	57.6	934-935 bp	1.0	52.6
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.4	57.6	932-933 bp	1.0	51.4
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	GCTGAGGTTATAGTTAAAGCTGC	50.0	43.5	58.6	57.6	933-934 bp	1.0	49.6
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	GCTGAGGTTATAGTTAAAGCTGC	45.0	43.5	57.4	57.6	940-941 bp	1.0	49.2