Hotspot

petA-psbJ

LSC · 66884-68015 · 1132 bp

Back to Triumfetta

Pi 0.00919

MarkerSeek score 48.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 26
pi: 0.00919
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGGACCGGAACTTCTTGTTT	ATCCCATTCGGAAGGATTGC	50.0	50.0	58.0	58.0	1565-1598 bp	1.0	82.2
petA-psbJ_p2	GGGACCGGAACTTCTTGTTT	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1729-1761 bp	1.0	81.7
petA-psbJ_p3	GGGACCGGAACTTCTTGTTT	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1826-1858 bp	1.0	81.7
petA-psbJ_p4	GGGACCGGAACTTCTTGTTT	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	2055-2087 bp	1.0	81.4
petA-psbJ_p5	GGGACCGGAACTTCTTGTTT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1824-1843 bp	0.857143	75.5