Hotspot

rbcL-accD

LSC · 60238-60948 · 711 bp

Back to Triumfetta

Pi 0.00309

MarkerSeek score 56.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 7
pi: 0.00309
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	GGACCAAGGCTATCCATTGA	55.0	50.0	58.0	57.0	889-894 bp	1.0	72.2
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	AGGACCAAGGCTATCCATTG	55.0	50.0	58.0	57.0	890-895 bp	1.0	72.1
rbcL-accD_p3	CACGCGAGGGTAATGAAGTT	GGACCAAGGCTATCCATTGA	50.0	50.0	58.3	57.0	915-920 bp	1.0	70.2
rbcL-accD_p4	CACGCGAGGGTAATGAAGTT	AGGACCAAGGCTATCCATTG	50.0	50.0	58.3	57.0	916-921 bp	1.0	70.2
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GGACCAAGGCTATCCATTGA	55.0	50.0	57.7	57.0	875-880 bp	1.0	70.2