Hotspot

trnF-GAA-ndhJ

LSC · 51364-52073 · 710 bp

Back to Triumfetta

Pi 0.0087
MarkerSeek score 57.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
710
variable_sites
18
pi
0.0087
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGCCCCCAATTTTTATGAAA 50.0 35.0 58.1 53.5 766-782 bp 1.0 47.8
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA TTGCCCCCAATTTTTATGAA 50.0 35.0 58.1 53.5 767-783 bp 1.0 47.8
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TTGCCCCCAATTTTTATGAAA 50.0 33.3 58.1 54.5 767-783 bp 1.0 47.0
trnF-GAA-ndhJ_p4 TAATGCGTCGGTAATGGTCG TGCCCCCAATTTTTATGAAA 50.0 35.0 58.2 53.5 821-837 bp 1.0 46.7
trnF-GAA-ndhJ_p5 TAATGCGTCGGTAATGGTCG TTGCCCCCAATTTTTATGAA 50.0 35.0 58.2 53.5 822-838 bp 1.0 46.7