Hotspot

psbZ-trnG-GCC

LSC · 38010-38743 · 734 bp

Back to Triumfetta

Pi 0.00987

MarkerSeek score 66.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 15
pi: 0.00987
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	891-993 bp	1.0	81.9
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	889-991 bp	1.0	81.9
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TACATTGACCTGGGCGGATA	50.0	50.0	57.8	58.2	927-1029 bp	1.0	81.5
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTT	TACATTGACCTGGGCGGATA	50.0	50.0	57.8	58.2	925-1027 bp	1.0	81.5
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	AAATACATTGACCTGGGCGG	50.0	50.0	57.8	58.2	930-1032 bp	1.0	81.2