Hotspot

trnS-GCU-trnS-CGA

LSC · 8347-9123 · 777 bp

Back to Triumfetta

Pi 0.0076

MarkerSeek score 58.10

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 14
pi: 0.0076
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	GCTCGGAATATGAATCAAACCG	50.0	45.5	58.0	57.9	892-916 bp	1.0	65.0
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	GCTCGGAATATGAATCAAACCG	50.0	45.5	58.0	57.9	891-915 bp	1.0	65.0
trnS-GCU-trnS-CGA_p3	GAAAGAGGGGGATTCGAACC	GCTCGGAATATGAATCAAACCG	55.0	45.5	58.0	57.9	943-967 bp	1.0	64.6
trnS-GCU-trnS-CGA_p4	GGAAAGAGGGGGATTCGAAC	GCTCGGAATATGAATCAAACCG	55.0	45.5	58.0	57.9	944-968 bp	1.0	64.6
trnS-GCU-trnS-CGA_p5	CGCACAACGGATTAGCAATC	GCTCGGAATATGAATCAAACCG	50.0	45.5	57.9	57.9	907-931 bp	1.0	64.3