Hotspot

rps16-trnQ-UUG

LSC · 6444-7053 · 610 bp

Back to Triumfetta

Pi 0.00562

MarkerSeek score 56.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 9
pi: 0.00562
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	704-731 bp	1.0	82.6
rps16-trnQ-UUG_p2	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	683-710 bp	1.0	82.5
rps16-trnQ-UUG_p3	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	681-708 bp	1.0	82.5
rps16-trnQ-UUG_p4	AGTGGTGGGTTTTGACATCC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.0	58.2	734-761 bp	1.0	81.7
rps16-trnQ-UUG_p5	CAGCCGATCATGTCCTTCAA	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.0	58.2	713-740 bp	1.0	81.6