Hotspot

trnL-trnI

IRa · 130423-131919 · 1497 bp

Back to Triticum

Pi 0.0234

MarkerSeek score 57.40

Primer available yes

Label trnL-trnI

Feature Metadata

label_name: trnL-trnI
feature_type: igs
region: IRa
length_bp: 1497
variable_sites: 87
pi: 0.0234
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnI_p1	AAATGGTAGACACGCGAGAC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1598-2484 bp	1.0	86.8
trnL-trnI_p2	TGAAATGGTAGACACGCGAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1600-2486 bp	1.0	86.8
trnL-trnI_p3	TGGTGAAATGGTAGACACGC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.2	58.0	1603-2489 bp	1.0	85.3
trnL-trnI_p4	GAAATGGTAGACACGCGAGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.7	58.0	1599-2485 bp	1.0	84.7
trnL-trnI_p5	GTTCGAGTCCTCTTCAAGGC	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.3	58.0	1550-2436 bp	1.0	84.7