Hotspot

ndhB-trnL

IRa · 129739-130341 · 603 bp

Back to Triticum

Pi 0.00381

MarkerSeek score 50.60

Primer available yes

Label ndhB-trnL

Feature Metadata

label_name: ndhB-trnL
feature_type: igs
region: IRa
length_bp: 603
variable_sites: 13
pi: 0.00381
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	632-663 bp	1.0	85.1
ndhB-trnL_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	630-661 bp	1.0	85.1
ndhB-trnL_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	627-658 bp	1.0	83.6
ndhB-trnL_p4	TGCAATTGCTCAGGATACCC	TTGAATGCGCATTCTCCGTA	50.0	45.0	57.9	58.0	735 bp	0.043478	41.1
ndhB-trnL_p5	TGCAATTGCTCAGGATACCC	GGGAATGCTCATTGAATGCG	50.0	50.0	57.9	57.9	746 bp	0.043478	40.2