Hotspot

trnL-ndhB

IRb · 84553-85155 · 603 bp

Back to Triticum

Pi 0.00365

MarkerSeek score 50.10

Primer available yes

Label trnL-ndhB

Feature Metadata

label_name: trnL-ndhB
feature_type: igs
region: IRb
length_bp: 603
variable_sites: 11
pi: 0.00365
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	632-663 bp	1.0	84.9
trnL-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	630-661 bp	1.0	84.9
trnL-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	627-658 bp	1.0	83.4
trnL-ndhB_p4	TTGAATGCGCATTCTCCGTA	TGCAATTGCTCAGGATACCC	45.0	50.0	58.0	57.9	735 bp	0.043478	41.1
trnL-ndhB_p5	GGGAATGCTCATTGAATGCG	TGCAATTGCTCAGGATACCC	50.0	50.0	57.9	57.9	746 bp	0.043478	40.2