Hotspot

trnI-trnL

IRb · 82975-84471 · 1497 bp

Back to Triticum

Pi 0.0237

MarkerSeek score 58.00

Primer available yes

Label trnI-trnL

Feature Metadata

label_name: trnI-trnL
feature_type: igs
region: IRb
length_bp: 1497
variable_sites: 93
pi: 0.0237
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-trnL_p1	GTTGGGCGCTTTAACCATTC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	1598-2484 bp	1.0	87.0
trnI-trnL_p2	GTTGGGCGCTTTAACCATTC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	1600-2486 bp	1.0	87.0
trnI-trnL_p3	GTTGGGCGCTTTAACCATTC	TGGTGAAATGGTAGACACGC	50.0	50.0	58.0	58.2	1603-2489 bp	1.0	85.5
trnI-trnL_p4	GTTGGGCGCTTTAACCATTC	GAAATGGTAGACACGCGAGA	50.0	50.0	58.0	57.7	1599-2485 bp	1.0	84.9
trnI-trnL_p5	GTTGGGCGCTTTAACCATTC	GTTCGAGTCCTCTTCAAGGC	50.0	55.0	58.0	58.3	1550-2436 bp	1.0	84.9