Hotspot

psbT-psbN

LSC · 70479-70526 · 48 bp

Back to Triticum

Pi 0.0242

MarkerSeek score 57.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 48
variable_sites: 7
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	217 bp	1.0	82.4
psbT-psbN_p2	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	212 bp	1.0	82.4
psbT-psbN_p3	AAGGTTCCACCAACTCCAAC	TCGCCATCTCCATATCTGGT	50.0	50.0	57.9	58.0	197 bp	1.0	82.2
psbT-psbN_p4	CCACCTAAGGTTCCACCAAC	TCGCCATCTCCATATCTGGT	55.0	50.0	58.1	58.0	203 bp	1.0	81.9
psbT-psbN_p5	TCTTCTTCCGAGAACCACCT	CCATTCGAAGAACACGGAGA	50.0	50.0	58.0	57.6	128 bp	1.0	81.4