Hotspot

trnS-psbD

LSC · 7996-8994 · 999 bp

Back to Triticum

Pi 0.00628

MarkerSeek score 57.00

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 22
pi: 0.00628
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1150-1194 bp	1.0	83.1
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1128-1172 bp	1.0	82.1
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1112-1156 bp	1.0	81.6
trnS-psbD_p4	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	55.0	55.0	57.8	58.1	1201-1245 bp	1.0	81.3
trnS-psbD_p5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	45.0	50.0	58.0	58.3	1143-1187 bp	1.0	81.3