Hotspot

rps16-trnQ

LSC · 5625-6685 · 1061 bp

Back to Triticum

Pi 0.0172

MarkerSeek score 55.80

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 100
pi: 0.0172
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	CCACAATCGATCGTGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	626-1156 bp	1.0	91.3
rps16-trnQ_p2	CCACAATCGATCGTGTCCTT	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.0	57.8	695-1225 bp	1.0	90.5
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	605-1135 bp	1.0	90.3
rps16-trnQ_p4	CCACAATCGATCGTGTCCTT	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.0	57.8	630-1160 bp	1.0	90.2
rps16-trnQ_p5	ATCCACAATCGATCGTGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	628-1158 bp	1.0	89.6