Hotspot

matK-trnK

LSC · 3216-3813 · 598 bp

Back to Triticum

Pi 0.00687

MarkerSeek score 54.00

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 29
pi: 0.00687
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	CCATAATCAGGGGCAAATGC	CCACGACTGATCCTCAAAGG	50.0	55.0	57.2	58.0	726 bp	0.043478	35.0
matK-trnK_p2	CCATAATCAGGGGCAAATGC	AAGACCACGACTGATCCTCA	50.0	50.0	57.2	58.1	730 bp	0.043478	34.5
matK-trnK_p3	CCATAATCAGGGGCAAATGC	AGACCACGACTGATCCTCAA	50.0	50.0	57.2	58.1	729 bp	0.043478	34.5
matK-trnK_p4	CCATAATCAGGGGCAAATGC	AGAAGACCACGACTGATCCT	50.0	50.0	57.2	57.8	732 bp	0.043478	33.3
matK-trnK_p5	CCATAATCAGGGGCAAATGC	GAAGACCACGACTGATCCTC	50.0	55.0	57.2	57.4	731 bp	0.043478	30.6