Hotspot

psbA-trnK

LSC · 1153-1384 · 232 bp

Back to Triticum

Pi 0.0152

MarkerSeek score 58.40

Primer available yes

Label psbA-trnK

Feature Metadata

label_name: psbA-trnK
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 31
pi: 0.0152
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK_p1	GCTTGTACTTTCGCGTCTCT	AAATGCAAGCACGATTTGGG	50.0	45.0	58.3	57.9	365-369 bp	1.0	86.3
psbA-trnK_p2	AAAACACCGAACCATCCGAT	AAATGCAAGCACGATTTGGG	45.0	45.0	57.8	57.9	436-440 bp	1.0	85.7
psbA-trnK_p3	CACCGAACCATCCGATGTAA	AAATGCAAGCACGATTTGGG	50.0	45.0	57.7	57.9	432-436 bp	1.0	84.9
psbA-trnK_p4	AAAACACCGAACCATCCGAT	CACGATTTGGGGAGAGGTTT	45.0	50.0	57.8	57.8	431 bp	0.043478	38.6
psbA-trnK_p5	GCTTGTACTTTCGCGTCTCT	CACGATTTGGGGAGAGGTTT	50.0	50.0	58.3	57.8	360 bp	0.043478	37.7