Hotspot

ycf1

IRb · 108998-114199 · 5202 bp

Back to Trithuria

Pi 0.0523

MarkerSeek score 67.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5202
variable_sites: 268
pi: 0.0523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACTCAAAAGCAGCACGAAG	GTCCTTTCTGTGCAGTTCCA	50.0	50.0	58.0	58.0	1220-1408 bp	1.0	87.3
ycf1_p2	ACTCAAAAGCAGCACGAAGA	GTCCTTTCTGTGCAGTTCCA	45.0	50.0	58.0	58.0	1219-1407 bp	1.0	87.2
ycf1_p3	CACTCAAAAGCAGCACGAAG	CCCTATCACCCCCTACTAGC	50.0	60.0	58.0	58.1	734 bp	0.5	59.3
ycf1_p4	CACTCAAAAGCAGCACGAAG	CCTATCACCCCCTACTAGCC	50.0	60.0	58.0	58.1	733 bp	0.5	59.3
ycf1_p5	GCCATGGAATTAGCCAGTCA	GTCCTTTCTGTGCAGTTCCA	50.0	50.0	57.9	58.0	1099 bp	0.5	59.3