Hotspot

ycf1-tRNA-Asn(GUU)

IRa · 140851-141336 · 486 bp

Back to Trithuria

Pi 0.377
MarkerSeek score 77.00
Primer available yes
Label ycf1-tRNA-Asn(GUU)

Feature Metadata

label_name
ycf1-tRNA-Asn(GUU)
feature_type
igs
region
IRa
length_bp
486
variable_sites
182
pi
0.377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-tRNA-Asn(GUU)_p1 GTAGCAAAGTGAACACGTGC CGGAAACAGATCCAGTGGAG 50.0 55.0 58.0 58.0 1186-1429 bp 1.0 90.4
ycf1-tRNA-Asn(GUU)_p2 GTAGCAAAGTGAACACGTGC CCGTTGTTCCTGAGTAGCTC 50.0 55.0 58.0 58.0 862 bp 0.5 59.8
ycf1-tRNA-Asn(GUU)_p3 GTAGCAAAGTGAACACGTGC CGTTGTTCCTGAGTAGCTCC 50.0 55.0 58.0 58.0 861 bp 0.5 59.8
ycf1-tRNA-Asn(GUU)_p4 GTAGCAAAGTGAACACGTGC GTTGTTCCTGAGTAGCTCCG 50.0 55.0 58.0 58.0 860 bp 0.5 59.8
ycf1-tRNA-Asn(GUU)_p5 GTAGCAAAGTGAACACGTGC GGCGCTTCCACATCTTCTTA 50.0 50.0 58.0 58.0 1252 bp 0.5 59.7