Hotspot

tRNA-Asn(GUU)-ycf1

IRb · 108512-108997 · 486 bp

Back to Trithuria

Pi 0.377

MarkerSeek score 77.00

Primer available yes

Label tRNA-Asn(GUU)-ycf1

Feature Metadata

label_name: tRNA-Asn(GUU)-ycf1
feature_type: igs
region: IRb
length_bp: 486
variable_sites: 182
pi: 0.377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn(GUU)-ycf1_p1	CGGAAACAGATCCAGTGGAG	GTAGCAAAGTGAACACGTGC	55.0	50.0	58.0	58.0	1186-1429 bp	1.0	90.4
tRNA-Asn(GUU)-ycf1_p2	CCGTTGTTCCTGAGTAGCTC	GTAGCAAAGTGAACACGTGC	55.0	50.0	58.0	58.0	862 bp	0.5	59.8
tRNA-Asn(GUU)-ycf1_p3	CGTTGTTCCTGAGTAGCTCC	GTAGCAAAGTGAACACGTGC	55.0	50.0	58.0	58.0	861 bp	0.5	59.8
tRNA-Asn(GUU)-ycf1_p4	GTTGTTCCTGAGTAGCTCCG	GTAGCAAAGTGAACACGTGC	55.0	50.0	58.0	58.0	860 bp	0.5	59.8
tRNA-Asn(GUU)-ycf1_p5	GGCGCTTCCACATCTTCTTA	GTAGCAAAGTGAACACGTGC	50.0	50.0	58.0	58.0	1252 bp	0.5	59.7