Hotspot

psbE-petL

LSC · 65741-66906 · 1166 bp

Back to Trithuria

Pi 0.0883

MarkerSeek score 82.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 92
pi: 0.0883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAGCGTTCTCCCGTA	AGCAGTCAGTAGAAAACCGA	50.0	45.0	57.9	56.5	1208-1239 bp	1.0	77.5
psbE-petL_p2	AAAAGAGCGTTCTCCCGTAC	AGCAGTCAGTAGAAAACCGA	50.0	45.0	57.9	56.5	1207-1238 bp	1.0	77.5
psbE-petL_p3	ACGAATGACCAACCTGCAAT	AGCAGTCAGTAGAAAACCGA	45.0	45.0	58.1	56.5	1290-1321 bp	1.0	77.0
psbE-petL_p4	TTCTCCCGTACTTCCAGACA	AGCAGTCAGTAGAAAACCGA	50.0	45.0	57.7	56.5	1198-1229 bp	1.0	75.8
psbE-petL_p5	CGAATGACCAACCTGCAATG	AGCAGTCAGTAGAAAACCGA	50.0	45.0	57.7	56.5	1289-1320 bp	1.0	75.6