Hotspot

petA-psbJ

LSC · 63865-64966 · 1102 bp

Back to Trithuria

Pi 0.109

MarkerSeek score 83.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 93
pi: 0.109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1004-1164 bp	1.0	84.9
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	TGTGATCGGTTTAGTAGGCG	50.0	50.0	58.2	57.4	1039-1199 bp	1.0	84.6
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	GTGATCGGTTTAGTAGGCGT	50.0	50.0	58.2	57.4	1038-1198 bp	1.0	84.6
petA-psbJ_p4	AGAAGGTTCAATTGGCCGAA	CATATTCCGGATTGGGCTCA	45.0	50.0	57.7	57.4	1003-1163 bp	1.0	84.0
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TGTGATCGGTTTAGTAGGCG	45.0	50.0	57.7	57.4	1038-1198 bp	1.0	83.8